TopVision Low Melting Point Agarose

TopVision Low Melting Point Agarose

Thermo Scientific Top Vision Low Melting Point Agarose is highly purified agarose with a low melting temperature certified by strict quality control test procedures.

Characteristics

Electroendosmosis (EEO)  – 0.11

Gel strength (1% gel) – 280 g/cm2

Gel strength (1.5% gel) – 1.165 g/cm2

Gel point (1.5% gel) – 25.9 °C

Melting point (1.5% gel) – 64.7 °C

Moisture – 3.88%

Applications

• Preparation of DNA preparative gels in routine molecular biology techniques.

• In-gel enzymatic processing (digestion, ligation etc.).

• The DNA recovered from agarose gels after electrophoresis can be used in enzymatic process (restriction, ligation, etc.) (1).

• Digestion of remelted agarose by agarase enzymes makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.

• May be used at concentrations between 0.8-2.0% with typical buffer systems.