VBP1 mouse monoclonal antibody, clone UMAB75, 100 uL/ 30 uL
R$8.629,20
| Imunógeno | Proteína recombinante humana de comprimento total de VBP1 humana (NP_003363) produzida na célula HEK293T. |
| Aplicações | IHC 1:100, |
| Aplicações2 | IHC |
| Resumo | A proteína codificada por este gene interage com a proteína Von Hippel-Lindau para formar um complexo intracelular. Por funcionar como uma proteína chaperona, suspeita-se que possa desempenhar um papel no transporte da proteína Von Hippel-Lindau dos grânulos perinucleares para o núcleo ou citoplasma. |
| Formulação | PBS (pH 7.3) contendo 1% BSA, 50% glicerol e 0.02% azida sódica. |
| Purificação | Purificado a partir de fluidos de ascite de camundongo por cromatografia de afinidade |
| Isotipo | IgG1 |
| Reatividade | Humano |
| Hospedeiro | Camundongo |
| Tamanho | 22.4 kDa |
| Tipo | UltraMAB |
| Concentração | 1.00mg/ml |
Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules
Sebastian Markmiller, Sahar Soltanieh, …, Gene W Yeo
“Abstract: Stress granules (SGs) are transient ribonucleoprotein (RNP) aggregates that form during cellular stress and are increasingly implicated in human neurodegeneration. To study the proteome and compositional diversity of SGs in different cell types and in the context of neurodegeneration-linked mutations, we used ascorbate peroxidase (APEX) proximity labeling, mass spectrometry, and immunofluorescence to identify ~150 previously unknown human SG components. A highly integrated, pre-existing SG protein interaction network in unstressed cells facilitates rapid coalescence into larger SGs. Approximately 20% of SG diversity is stress or cell-type dependent, with neuronal SGs displaying a particularly complex repertoire of proteins enriched in chaperones and autophagy factors. Strengthening the link between SGs and neurodegeneration, we demonstrate aberrant dynamics, composition, and subcellular distribution of SGs in cells from amyotrophic lateral sclerosis (ALS) patients”
