Inter- and intra-tumor heterogeneity of metastatic prostate cancer determined by digital spatial gene expression profiling
Lauren Brady, Michelle Kriner, …, Peter S Nelson
“Digital spatial profilingPrepared slides were stained with immunofluorescent antibodies to facilitate the identification of tissue morphology: pan-cytokeratin (AE1 + AE3, Novus Biologicals) for epithelial cells and CD3 (UMAB54, OriGene labeled with Alexi Fluor 647) or CD45 (2B11 + PD7/26, Novus Biologicals) for T cells, as well as the DNA stainSyto13 (NanoString, 121303303). Stained slides were loaded onto a GeoMx instrument and scanned. One circular ROI measuring 500 μm in diameter was selected”




