Dissolved molecular hydrogen (H2) in Peritoneal Dialysis (PD) solutions preserves mesothelial cells and peritoneal membrane integrity
Masaaki Nakayama, Wan-Jun Zhu, …, Sadayoshi Ito
“, TX), proliferative marker Ki-67 (Novus Biologicals, CO), apoptosis marker M30 CytoDeath (PEVIVA, Sundbyberg, Sweden), total macrophage CD68 mouse anti-rat monoclonal (ED1) antibody (LSBio, Seattle, WA), M1 macrophage CD80 mouse monoclonal antibody (Origene Technologies Inc., Rockville, MD), and M2 macrophage CD163 mouse monoclonal antibody (Leica Biosystems, Nussloch, Germany). For quantitative analysis, the number of positive cells in the peritoneal tissue sample was counted in five randomly “