CDH2 mouse monoclonal antibody, clone UMAB23, 100 uL/ 30 uL
R$8.629,20
| Imunógeno | Proteína recombinante humana de comprimento total de CDH2 humano (NP_001783) produzida na célula HEK293T. |
| Aplicações | WB 1:2000, IHC 1:150, FLOW 1:100, |
| Aplicações2 | WB, IHC, FC |
| Resumo | Este gene é uma caderina clássica da superfamília das caderinas. A proteína codificada é uma glicoproteína de adesão célula-célula dependente de cálcio composta por cinco repetições de caderina extracelular, uma região transmembranar e uma cauda citoplasmática altamente conservada. A proteína funciona durante a gastrulação e é necessária para o estabelecimento da assimetria esquerda-direita. Em certas sinapses do sistema nervoso central, a adesão pré-sináptica a pós-sináptica é mediada pelo menos em parte por este produto gênico. |
| Formulação | PBS (pH 7.3) contendo 1% BSA, 50% glicerol e 0.02% azida sódica. |
| Purificação | Purificado a partir de fluidos de ascite de camundongo por cromatografia de afinidade |
| Isotipo | IgG2a |
| Reatividade | Humano |
| Hospedeiro | Camundongo |
| Tamanho | 97.2 kDa |
| Tipo | UltraMAB |
| Concentração | 1.1mg/ml |
Cathepsin B defines leader cells during the collective invasion of salivary adenoid cystic carcinoma
Jia-Shun Wu, Zhu-Feng Li, …, Ya-Ling Tang
“The sections were autoclaved for antigen retrieval and blocked with 3% hydrogen peroxide and goat serum albumin, followed by routine immunohistochemical staining procedures, as previously described ( , ).. The primary antibodies used were as follows: Rabbit anti-E-cadherin, 1:200 (cat. no. UM870076; OriGene Technologies, Inc., Shanghai, China); mouse anti-N-cadherin, 1:200 (cat. no. UM500023; OriGene Technologies, Inc.); rabbit anti-vimentin, 1:200 (cat. no. UM800054CF; OriGene Technologies, Inc.); and rabbit anti-CTSB, 1:50 (cat. no. ab230401; Abcam, Cambridge, MA, USA).. Within each tumor, the cancer cells that were closest to the connective tissue or other normal tissue (such as the salivary glands and nerves) were defined as the invasive tumor front and the other tumor areas were regarded as the central.. “
Macrophages significantly enhance wound healing in a vascularized skin model.
Franziska Kreimendahl, Yvonne Marquardt, …, Jens Malte Baron
“Abstract: Tissue-engineered dermo-epidermal skin grafts could be applied for the treatment of large skin wounds or used as an in vitro wound-healing model. However, there is currently no skin replacement model that includes both, endothelial cells to simulate vascularization, and macrophages to regulate wound healing and tissue regeneration. Here, we describe for the first time a tissue-engineered, fully vascularized dermo-epidermal skin graft based on a fibrin hydrogel scaffold, using exclusively human primary cells. We show that endothelial cells and human dermal fibroblasts form capillary-like structures within the dermis whereas keratinocytes form the epithelial cell layer. Macrophages played a key role in controlling the number of epithelial cells and their morphology after skin injury induced with a CO2 laser”
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