” followed by an additional wash with PBS for 5 min. Tissues were subsequently probed with primary antibodies for 1 h at room temperature. The following primary antibodies were used: Anti-CD3 (cat. no. LN10; 1: 50–100 depending on the tissue samples; OriGene Technologies, Inc.), anti-CD56 (cat. no. UMAB83; 1:100; OriGene Technologies, Inc.), anti-CD20 (cat. no. L26; 1:100; Dako; Agilent Technologies, Inc.), anti-TIA-1 (cat. no. 2G9A10F5; 1:100; OriGene Technologies, Inc.), anti-granzyme B (cat. no”
“Histopathology and immunohistochemistrySurgical samples were collected and embedded with paraffin for histological and immunohistochemical analyses. Immunohistochemical analyses for synaptophysin (SYN) (rabbit monoclonal antibody, OriGene, Rockville, MD, USA), chromogranin A (CGA; mouse monoclonal antibody; OriGene), cluster of differentiation (CD56; mouse monoclonal antibody; OriGene), cytokeratin (CK; mouse monoclonal antibody; OriGene), vimentin (VIM; mouse monoclonal antibody; OriGene), S-10”.
” incubation. The primary antibodies used were anti-HA (Roche) as well as, where available, primary antibodies against the native human genes. The primary antibody concentration used was 1 μg/μl for the protein specific antibodies (anti-Ncam1, UMAB83, Origene and anti-Nsf, ab16681, Abcam) and a 1:1000 dilution for the mouse anti-HA. Membranes were incubated with the secondary antibody (GE Healthcare) at a 1:5000 concentration for one hour, followed by signal detection using the Amersham ECL system”.