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“ChIP assays were performed using Chromatin Immunoprecipitation kit (Chromatin, USA) according to the manufacturer’s protocols.. Immunoprecipitation reactions were performed with 5 μg antibody against S100P (OriGene #UM500024, USA) or with IgG, used as a negative control.. Purified DNA was suspended subsequently for following qRT-PCR analysis using primers of SLC2A5 promoter.”




