R$20.791,29
O In-Fusion Snap Assembly Master Mix permite clonagem direcional de alta eficiência e alta fidelidade de um ou mais fragmentos de PCR em qualquer vetor. Além do kit de clonagem, este pacote inclui:
– Um kit NucleoSpin Gel e PCR Clean-Up: Este kit é adequado para extração de gel, bem como purificação de PCR. Os kits são fornecidos com colunas de purificação individuais.
– Stellar Competent Cells: Células competentes de alta eficiência são essenciais para o sucesso da In-Fusion Cloning. Uma cepa E. coli HST08 está incluída, o que fornece alta eficiência de transformação (maior que 5 x 10^8 cfu/µg) e complementa a eficiência de todos os kits In-Fusion Snap Assembly. As células são fornecidas em alíquotas de 100 μl em tubos individuais.
– PrimeSTAR Max DNA Polymerase: Esta conveniente mistura mestre líquida 2X oferece amplificação de DNA excepcionalmente precisa, eficiente e rápida. A pré-mistura contém dNTPs e um tampão otimizado, permite a configuração rápida de reações de PCR e facilita a clonagem bem-sucedida. A mistura mestre de polimerase é fornecida em alíquotas de 625 μl.
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In-Fusion Cloning
In-Fusion Cloning kits are unique in their ability to allow easy and accurate seamless cloning without the use of ligase. In just one quick reaction, you can clone any insert into any vector at any locus, without the hassle of subcloning, unwanted extra bases, or inconvenient restriction sites. Watch our videos to learn more »
Figure 1. In-Fusion Cloning protocol. The key to In-Fusion Cloning is 15 bp of homology between your insert(s) and linearized vector backbone.
In-Fusion Cloning is beautifully versatile. With an easy-to-follow protocol that provides high efficiency, you can successfully perform a wide array of applications, all with the same kit. Clone your insert specifically and directionally, and get an exceptionally high rate of cloning accuracy in every experiment.
The homologous ends between insert(s) and vector are designed into your primers, and the two are annealed during the In-Fusion Cloning reaction. Because there is no need to digest the insert(s) prior to incubation with the vector, the In-Fusion Cloning workflow is significantly shorter than that of traditional cloning by restriction enzymes. This technology does not require a ligation step either, saving even more time and getting you to the transformation step sooner. With empty vector background levels approaching zero, you can pick just a few colonies and be confident that you have the right clone. Cloning shouldn’t be work; it should just work!
Figure 2. In-Fusion Cloning and traditional cloning workflows. The In-Fusion Cloning protocol is simple and elegant. Just four simple steps guarantee cloning accuracy above 95%.
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