Purified KI67 (MKI67) mouse monoclonal antibody, clone UMAB107, 100 uL/ 30 uL
R$8.063,10
| Imunógeno | Fragmento de proteína recombinante humana correspondente aos aminoácidos 1160-1493 de MKI67 humano (NP_002408) produzido em E. coli. |
| Aplicações | IHC: 1:300 |
| Aplicações2 | IHC |
| Resumo | Este gene codifica uma proteína nuclear que está associada e pode ser necessária para a proliferação celular. Variantes de transcrição com splicing alternativo foram descritas. Existe um pseudogene relacionado no cromossomo X. |
| Formulação | PBS (pH 7.3) contendo 1% BSA, 50% glicerol e 0.02% azida sódica. |
| Purificação | Purificado a partir de fluidos de ascite de camundongo por cromatografia de afinidade |
| Isotipo | IgG2a |
| Reatividade | Humano |
| Hospedeiro | Camundongo |
| Tamanho | |
| Tipo | UltraMAB |
| Concentração | 1.00mg/ml |
“Antigen retrieval was performed in a microwave by placing the sections in epitope retrieval solution (0.01 M citrate buffer, pH 6.0) for 20 minutes; endogenous peroxidase was inhibited by immersing the sections in 0.3% hydrogen peroxide for 10 minutes.. Sections were then incubated with the primary mouse monoclonal antibody to BCAT1 (BD Biosciences), mouse monoclonal antibody to human IDH1 R132H (Dianova), mouse monoclonal antibody to human KI67 (UM800033, ORIGENE), goat polyclonal antibody to rat CD34 (R & D Systems), or mouse monoclonal antibody to human HIF-1 alpha (Thermo) in Dako REAL antibody diluent (Dako).”
“CM073C; 1:200 dilution; Biocare Medical LLC, Concord, CA, USA), actin (mouse monoclonal antibody; catalog no. 202M-96; 1:150 dilution; Cell Marque, Sigma-Aldrich), HMB-45 (mouse monoclonal antibody; catalog no. Z-2088; 1:150 dilution; Zeta Co., Ltd.), melan-A (mouse monoclonal antibody; catalog no. Z-2052; 1:200 dilution; Zeta Co., Ltd.) and myoglobin (mouse monoclonal antibody; catalog no. NCL-MYOGLOBIN; 1:150 dilution; Leica Microsystems, Ltd.). … The Ki-67 index (mouse monoclonal antibody; catalog no. UM800033; 1:200 dilution; Origene Techynologies Inc.) was ~70% ( ).. Taken together, the histomorphological, immunohistochemical and MRI findings supported a diagnosis of LCS.”
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