“Immunohistology and Islet Cell Area QuantificationImmunostaining was performed on 5- to 7-mm formalin-fixed, paraffin- embedded (FFPE) mouse tissue sections using citrate-buffered boiling epitope retrieval.. The following antibodies were used: guinea pig anti-Insulin (AB7842), mouse anti-Glucagon (G2654), rabbit anti-CD3 (RM9107S), rabbit anti-GFP (AB183734), rabbit anti-Glucagon (AB92517), rabbit anti-pS6 (5364S), rabbit anti-SLC38A4 (LS-C31936), rabbit anti-SLC38A5 (AB72717), rabbit anti-SLC7A8 (Origene, UM500058), rat anti-MKI67 (14-5698-82), and appropriate Alexa Fluor 488-, 555-, 594-, or 647-labeled secondary antibodies. Nuclei were stained with Hoechst 33342 (#H21492).”
SLC7A8 mouse monoclonal antibody, clone UMAB70, 100 uL/ 30 uL
R$12.423,26
| Imunógeno | Proteína recombinante humana de comprimento total de SLC7A8 humana (NP_036376) produzida na célula HEK293T. |
| Aplicações | IHC 1:100, |
| Aplicações2 | IHC |
| Resumo | |
| Formulação | PBS (pH 7.3) contendo 1% BSA, 50% glicerol e 0.02% sódio azido. |
| Purificação | Purificado a partir de fluidos de ascite de camundongo por cromatografia de afinidade |
| Isotipo | IgG1 |
| Reatividade | Humano |
| Hospedeiro | Camundongo |
| Tamanho | 58.2 kDa |
| Tipo | UltraMAB |
| Concentração | 1.00mg/ml |
Elevated Serum Amino Acids Induce a Subpopulation of Alpha Cells to Initiate Pancreatic Neuroendocrine Tumor Formation
Derek K Smith, Lance Kates, …, Andrew S Peterson
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Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell
Danusa Menegaz, D Walker Hagan, …, Edward A Phelps
“polypeptide (Abcam #ab113694), rb-α-GABA (Sigma-Aldrich #A2052), ms-α-GAD65 C-term (in-house RRID:AB_528264), ms-α-GAD65 N-term (Christiane Hampe Lab), rb-α-GAD65 (Synapic Systems #198102), gp-α-GAD65 (Synaptic Systems #198104), rb-α-TauT (Sigma-Aldrich #HPA015028), rb-α-VGAT Oyster 650-labeled (Synaptic Systems #131103C5), rb-α-synaptophysin (Abcam #ab14692), rb-α-SV2C (Synaptic Systems #119202), rb-α−4F2HC (Santa Cruz Biotech. … #sc9160), ms-α-LAT2 (OriGene #UM500058), rb-α-Bestrophin 1 (Abcam #ab14928), rb-α-GAT1 (Synaptic Systems #274102), rb-α-GAT2 (Abcam #ab2896), rb-α-GAT3 (Synaptic Systems #274303), rb-α-GCP60 (Novus Biologicals # NBP1–83379), rb-α-taurine (Sigma-Aldrich #AB5022), Alexa Fluor conjugated secondary antibodies (Thermo Fisher).. #sc9160), ms-α-LAT2 (OriGene #UM500058), rb-α-Bestrophin 1 (Abcam #ab14928), rb-α-GAT1 (Synaptic Systems #274102), rb-α-GAT2 (Abcam #ab2896), rb-α-GAT3 (Synaptic Systems #274303), rb-α-GCP60 (Novus Biologicals # NBP1–83379), rb-α-taurine (Sigma-Aldrich #AB5022), Alexa Fluor conjugated secondary antibodies (Thermo Fisher).”
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