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Water, Molecular Biology Grade, DNase, RNase, Protease free, 100ml

Para aplicações críticas envolvendo nucleotídeos como RNA e DNA, use água de grau de biologia molecular HyClone sem nuclease, que não contém DEPC ou outros produtos químicos cancerígenos.




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SKU: SH30538.01 Categoria: Tag:

HyClone™ Water, Molecular Biology Grade

For critical applications involving nucleotides such as RNA and DNA, use nuclease-free Cytiva HyClone Molecular Biology-Grade Water, containing no DEPC or other carcinogenic chemicals.

Molecular Biology Grade Water – 100 mL


Item Description HyPure Molecular Biology Grade Water, deionized, distilled; no detectable nucleases or proteases
pH 5.0 to 7.0
Format Liquid
Quantity 100 mL
Storage Requirements 2° to 30° C
Shelf Life 24 months
Endotoxin Concentration ≤ 0.05 EU/mL
Sterilization 0.1 um sterile filtered
Certifications/Compliance Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities


Product Overview


Nuclease-free water is certified to have no detectable contaminating activity in DNase, RNase, and protease assays

Maximum allowable limit of endotoxin is 0.05 EU/mL

Purification process includes continuous deionization, reverse osmosis, ultraviolet light treatment, distillation, and 0.1µm filtration


Buffer preparation

DNA/RNA purification

DNA sequencing and libraries

Gene mapping

Recombinant DNA and cloning

PCR analysis

Northern Blot

Southern Blot


In order to work with nucleotides such as RNA and DNA and to prevent subsequent degradation due to enzymatic activity, it is necessary to use reagents, including water, that are devoid of all nucleases. Nucleases are naturally occurring enzymes whose role is specifically to cleave nucleotide polymeric molecules such as DNA or RNA at various points. Theoretically, just one molecule of a nuclease could systematically facilitate the destruction of an entire nucleotide sample over time.


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