For critical applications involving nucleotides such as RNA and DNA, use nuclease-free Cytiva HyClone Molecular Biology-Grade Water, containing no DEPC or other carcinogenic chemicals.

Product Overview

Quality:
Nuclease-free water is certified to have no detectable contaminating activity in DNase, RNase, and protease assays
Maximum allowable limit of endotoxin is 0.05EU/mL
Purification process includes continuous deionization, reverse osmosis, ultraviolet light treatment, distillation, and 0.1µm filtration

Applications:
Buffer preparation
DNA/RNA purification
DNA sequencing and libraries
Gene mapping
Recombinant DNA and cloning
PCR analysis
Northern Blot
Southern Blot

FOR FURTHER MANUFACTURING OR RESEARCH USE.  NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

In order to work with nucleotides such as RNA and DNA and to prevent subsequent degradation due to enzymatic activity, it is necessary to use reagents, including water, that are devoid of all nucleases. Nucleases are naturally occurring enzymes whose role is specifically to cleave nucleotide polymeric molecules such as DNA or RNA at various points. Theoretically, just one molecule of a nuclease could systematically facilitate the destruction of an entire nucleotide sample over time.